Journal: Lung cancer (Amsterdam, Netherlands)

Article Title: Combination therapy with anti-programmed cell death 1 antibody plus angiokinase inhibitor exerts synergistic antitumor effect against malignant mesothelioma via tumor microenvironment modulation.

doi: 10.1016/j.lungcan.2023.107219

Figure Lengend Snippet: Fig. 1. MPM cells secreted multiple angiogenic factors. A, Comprehensive analysis of the expression of transcripts of multiple angiogenic factors (VEGFA, PDGFb, FGF2, HGF, ANG1 and IL-8) in human umbilical vein endothelial cell (HUVEC), mesothelial cell (Met-5A) and MPM cell lines (MSTO-211H, H2452, H2052, and H28) by qRT-PCR. Expression of FGF2 transcripts in all MPM cells was higher than in HUVECs. B, Comprehensive analysis of the protein expression of angiogenic factor receptors (VEGFR2, PDGFRβ, FGFR1, c-Met, Tie2 and CXCR1) in HUVEC, human mesothelial cell and MPM cells by immunoblotting. MPM cells expressed higher level of FGFR1 compared with HUVECs. C, Antiproliferative effect of multi-angiokinase inhibitor nintedanib (NTD) in MPM cells. Mesothelial cells and MPM cells were treated with serially diluted NTD for up to 72 h. The relative numbers of viable cells were quantified using CCK-8 assay. Points, mean % viable cells; bars, SEM of at least three independent experiments performed in triplicate.

Article Snippet: Rabbit polyclonal Ab against CXC motif chemokine receptor 1 (CXCR1) (#55450–1- AP) was obtained from Proteintech (Rosemont, IL).

Techniques: Expressing, Quantitative RT-PCR, Western Blot, CCK-8 Assay

Journal: Canadian Journal of Veterinary Research

Article Title: Does antimicrobial therapy improve outcomes in horses with severe equine asthma and a positive tracheal wash bacterial culture?

doi:

Figure Lengend Snippet: There was no difference in interleukin-8 (IL-8) concentration of the bronchoalveolar lavage (BAL) fluid supernatant between the principal (n = 6) and control (n = 5) groups. Each horse is represented by a letter on the graph and the medians are shown as a line.

Article Snippet: Bronchoalveolar lavage fluid (BALF) supernatant was thawed in 1-mL aliquots for use in commercially available enzyme-linked immunosorbent assays (ELISA) for TNF-α [DuoSet ELISA Equine TNF-α (R&D Systems, Minneapolis, Minnesota, USA)] and IL-8 (Horse IL-8 ELISA Kit; Cusabio, College Park, Maryland, USA).

Techniques: Concentration Assay